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2.15 Glutathione peroxidase (GSH-Px) The reaction mixture containing 50 mM potassium phosphate, 1 mM EDTA, cytosol sample, 10 mM GSH, GSSG reductase (2.4 U/mL) and 1.5 mM NADPH was incubated at 37°C for 5 min, followed by the addition of tert-butylhydroperoxide