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In addition, DTP cells are capable of secreting immunosuppressive factors, including TGF-β and IL-10, which collectively create an immunosuppressive microenvironment, attracting the aggregation of immunosuppressive cells including myeloid-derived suppressor cells (MDSCs) and regulatory T cells (96–100)

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The cells were then washed three times with PBS containing 1% BSA (w/v) followed by 1 h incubation with 1:1000 anti-mouse Alexa Fluor 488 conjugated secondary antibody (diluted with PBS containing 5% BSA, Invitrogen) at 4 °C in the dark