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The reaction was quenched with 1% formic acid, samples were spun at 21,000g for 10 min to remove debris and undigested protein, then subjected to C18 solid-phase extraction (Sep-Pak, Waters) and vacuum centrifuged to near-dryness In preparation for TMT labelling, desalted peptides were dissolved in 200 mM HEPES pH 8.5
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