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To analyse DELE1-containing and HRI-containing protein assemblies in cytosolic extracts by native gel electrophoresis, cells were seeded and treated as described in the figure legends, washed with PBS and lysed for 20 min on ice with NativePAGE sample buffer (Thermo Fisher) supplemented with protease inhibitors and 0.02% digitonin (Sigma-Aldrich, D141)

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doi: 10.1016/j.neuropharm.2013.03.025 216 TuoQZZhangSTLeiP