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Blastocyst formation was assessed on day 7 (168 h) following IVF, and blastocysts were classified according to the degree of expansion and hatching status, as previously described (25): early blastocyst (small blastocyst with a blastocoel equal to or less than half of the embryo volume), expanded blastocyst (a large blastocyst with a blastocoel greater than half of the embryo volume or blastocyst with a blastocoel completely filling the embryo) and hatched blastocyst (hatching or already hatched blastocyst)
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Unravelling 5-oxoprolinuria (pyroglutamic aciduria) due to bi-allelic OPLAH mutations: 20 new mutations in 14 families
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