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The cells were permeabilized and blocked with 0.1% Triton-X-100 and 10% horse serum for 1 h, washed with 1% horse serum, 0.1% Triton-X-100, 1% BSA in 1 × PBS for 5 min

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Subsequently, the prepared EV stock solution was diluted with complete medium and applied to the RAW264.7 cell cultures at various concentrations