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We then performed a principal component analysis (PCA) on the normalized variables to obtain a reduced number of variables which was used as input for linear discriminant analyses (LDAs) to visualize the differences between strain groups across temperatures and sampling times

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In diet-induced obese mice lacking RAMP1 and RAMP3, cagrilintide’s potency was reduced and its activation of area postrema neurons was 57% lower than in wild-type mice, which the investigators interpreted as dependence on AMY1 and AMY3 receptors (Carvas et al., 2025)