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For instance, succinate and α-ketoglutarate jointly regulate pro- and anti-inflammatory transcriptional programs, while fatty acid oxidation modulates HIF-1α activity and ROS production, creating a metabolic–immune feedback loop that sustains inflammatory microenvironments

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Following gel de-staining, the glycans were enzymatically removed and fluorescently labeled following standard in-gel PNGase F and labeling protocols as previously described ( Supplemental Figure 1A