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To determine the expression of translocated Nrf2 and NF-κB in the nucleus, and the levels of Nrf2, Keap1, heme oxygenase-1 (HO-1) and p-IκBα in the cytoplasm, nuclear and cytoplasmic extracts from the lung tissue were prepared with a previously described method (Rajendrasozhan et al., 2010)

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