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Frontiers | Iron Metabolism and Ferroptosis in Epilepsy

Then, membranes were incubated overnight in 4 °C with primary antibodies: anti-GSDMD (abcam, ab209845, 1:1000), anti-GSDME (abcam, ab215191, 1:1000), anti-caspase-1 (Adipogen, AG-20B-0042-C100, 1:1000), anti-caspase-3 (Cell Signaling, 9662S, 1:1000), anti-cleaved caspase-3 (Cell Signaling, 9661S, 1:1000), anti-caspase-7 (Cell Signaling, 9492S, 1:1000), anti-cleaved caspase-7 (Cell Signaling, 9491S, 1:1000), anti-caspase-8 (AdipoGen, AG-20T-0137-C100, 1:1000), anti-RIP3 (Cell Signaling, 95702S, 1:1000), anti-phospho-RIP3 (Cell Signaling, 91702S, 1:1000), anti-MLKL (AdipoGen, AP14272B, 1:1000), anti-phospho-MLKL (Cell Signaling, 37333S, 1:1000), anti-phospho-MLKL Ser358 D6H3V (Cell Signaling, 91689S, 1:1000), anti-MLKL E7V4W (Cell Signaling, 26539S, 1:1000), anti-ZBP1 (AdipoGen, AG-20B-0010-C100, 1:1000), anti-LDHA-specific rabbit polyclonal (Proteintech, 19987-1-AP, 1:1000), anti-HMGB1 (abcam, ab18256, 1:1000), anti-GAPDH (Cell Signaling, 5174S, 1:1000), anti-β-actin (Cell Signaling, 8457S, 1:1000), anti-GPX4 (Abclonal, A1933, 1:1000), anti-thioredoxin 1 (Cell Signaling, C63C6, 1:1000) and anti-SAT1 (Proteintech, 10708-1-AP, 1:1000)

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Milanski et al.2009)