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SPR showed early titer advantages ( Figure 4 ), but C57BL/6 mice naturally exhibit Th1-biased responses with preferential IgG2c production even in the absence of Th1-promoting adjuvants ( Supplementary Figure S5 ), potentially reflecting affinity maturation processes

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In summary, our results suggest that glutathione metabolism plays an essential role in PDAC aggressiveness, supporting CSC survival, self-renewal and chemoresistance