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On the other hand, in vitro studies face limitations in understanding the physiologic functions of glutathione because most experiments are performed under a culture with atmospheric oxygen (~21%), which is about one order higher than in vivo oxygen conditions (2–5%)

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5.1.3 Neurotraumatic diseases 5.1.3.1 Spinal cord injury In recent years, researchers from the International Collaboration on Repair Discoveries at the University of British Columbia, Canada (130–132), applied EODF to treat rats with acute incomplete cervical and thoracic spinal cord injury (SCI), obtaining positive results in behavioral assessments and evaluations of neuroprotective effects through blood β -hydroxybutyrate levels and BDNF levels