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The raw spectra were searched against the fasta file containing the complete mouse reference proteome (UniProt, UP000000589, November 2022, 55,275 entries) for mouse tissue samples and C2C12 myotubes and against the complete human reference proteome (UniProt, UP000005640, November 2022, 82 492 entries) for HepG2 cells, with a precursor m/z range of 400 to 1250, and trypsin was chosen as the digestion enzyme
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