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Figure 9 illustrates this point: assuming two different protein–protein complexes, A–B and X–Y with similar energies of their bound state but different energies of their free states, any model considering only the bound state will predict similar binding affinities for those two complexes while, experimentally, they will have different affinities owing to the differences in their respective free states, The free state contribution is typically neglected in docking

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Mechanotransduction represents cellular responses to mechanical forces, translating such factors into chemical or electrical signals which alter the activation of various cell signaling pathways