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A master mix of the working reagent (WR), sufficient for all samples and standards, was prepared (105 μL 1X assay buffer, 1 μL GR enzyme, 0.25 μL NADPH and 0.5 μL DTNB per reaction)
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i , Plots demonstrating specificity of IL-10 and TGFβ antibodies used for staining on CX3CR1 + Ly6C + Mdcs
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