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Then, the RAW264.7 cells were lysed using RIPA lysis buffer supplemented with 1 mM phenylmethanesulfonyl fluoride (PMSF)
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Because of the reduced binding affinity, IGF-1 LR3 has a 5 to 10-fold greater potency and helps us to understand the complex biology of the IGF system better [6, 8]
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