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In addition to PGC-1α and SIRT1, AMPK can phosphorylate a number of different transcription factors, including Nrf1/2 and TFAM (67)
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Preparative HPLC was performed to isolate the targeted compounds, mainly F1–F8, with the help of the TLC and HPLC-UV/DAD above used for monitoring and confirmation, respectively
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