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Crisper CAS9 gene-editing in BEAS-2B transformed cells Double sgRNA array cloning: The target vector pAAV sgRNAXhoI-EFS-SpCas9-P2A-Puro (sequence available in this paper S6) was generated using a gBlock (IDT) containing the EFS short promoter, a P2A cleavage site and the Puromycin resistance gene
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