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For immunoprecipitation (IP) of methylated DNA, 1 μg (30 μL) of sonicated and denatured DNA sample was mixed with 20 μL of 10 x IP buffer (100 mM sodium phosphate buffer pH 7.0, 1.4 M NaCl, 0.5% Triton X-100), 2 μL of anti-5mC antibody (Cell Biolabs, San Diego, CA, USA) and RNase free water up to 200 μL

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