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Using siRNA against various forms of the G protein-coupled receptor kinase (GRK) family, DAMGO was demonstrated to phosphorylate T370 and S375 by GRK2 and 3 while morphine increases S375 phosphorylation by GRK5 (Doll et al., 2012)
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Finally, the solution was dried in an oven at 80°C for 12 h and then calcined at 400°C for 3 h in a muffle furnace
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