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Cellular Thermal Shift Assay (CETSA) HEK293T cells cultured in 100 mm × 20 mm tissue culture dishes were treated with media containing DMSO or GPX4 activator for 3 h at 37°C in a tissue culture incubator (with 5% CO 2 )
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5), and proteomic analysis did not reveal downregulation of GPX4 or upregulation of ACSL4, two characteristic markers of ferroptosis (Supplementary Fig
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